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pmxs got1  (Addgene inc)
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Addgene inc pmxs got1
(Left panel) Under OXPHOS-competent conditions, aspartate is normally produced by GOT2 in the mitochondria. dmaKG can increase intracellular aKG levels and promote mitochondrial metabolism to maintain aspartate levels. (Right panel) Under OXPHOS-incompetent conditions, cells rely on <t>GOT1</t> in the cytosol for aspartate synthesis. Aberrant elevation in aKG levels can result in nitrogen trapping (converting aKG to Glu) via GOT1, leading to aspartate exhaustion. Blue arrows depict the mode of action of dmaKG. Glu, glutamate; OAA, oxaloacetate.
Pmxs Got1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(Left panel) Under OXPHOS-competent conditions, aspartate is normally produced by GOT2 in the mitochondria. dmaKG can increase intracellular aKG levels and promote mitochondrial metabolism to maintain aspartate levels. (Right panel) Under OXPHOS-incompetent conditions, cells rely on GOT1 in the cytosol for aspartate synthesis. Aberrant elevation in aKG levels can result in nitrogen trapping (converting aKG to Glu) via GOT1, leading to aspartate exhaustion. Blue arrows depict the mode of action of dmaKG. Glu, glutamate; OAA, oxaloacetate.

Journal: Cancer research

Article Title: Nitrogen trapping as a therapeutic strategy in tumors with mitochondrial dysfunction

doi: 10.1158/0008-5472.CAN-20-0246

Figure Lengend Snippet: (Left panel) Under OXPHOS-competent conditions, aspartate is normally produced by GOT2 in the mitochondria. dmaKG can increase intracellular aKG levels and promote mitochondrial metabolism to maintain aspartate levels. (Right panel) Under OXPHOS-incompetent conditions, cells rely on GOT1 in the cytosol for aspartate synthesis. Aberrant elevation in aKG levels can result in nitrogen trapping (converting aKG to Glu) via GOT1, leading to aspartate exhaustion. Blue arrows depict the mode of action of dmaKG. Glu, glutamate; OAA, oxaloacetate.

Article Snippet: Chandel (Northwestern University), and they were cultured in the standard medium supplemented with 0.1 mg/mL uridine ( 17 ). pMXs-GOT1 and pMXs-IRES-blasticidin empty vector were introduced into UOK262, 143B-wt and 786-O by viral transduction to generate stable lines. pMXs-SLC1A3 (Addgene, #72873) and pMXs-GOT1 (Addgene, #72872) were gifts from David Sabatini ( 15 ).

Techniques: Produced